Transcriptome analysis reveals class IX ethylene response factors show specific up-regulation in resistant but not susceptible Medicago truncatula lines following infection with Rhizoctonia solani

摘要: The fungal pathogen Rhizoctonia solani AG8 causes substantial losses to cereal and legume production in Australia the Pacific Northwest of United States America. Mutant analyses have revealed a critical role for ethylene mediated defence signalling resistance R. model Medicago truncatula which is, at least part, by dependent accumulation isoflavonoids. In this study we investigate potential members response transcription factor (ERF) family mediating isoflavonoid response. A strong early Rhizoctonia-responsive expression pattern was observed many class IX ERFs moderately resistant wild type line A17, while insensitive highly susceptible mutant sickle (skl) showed very limited regulation class. Conversely, skl demonstrated up-regulation II known act as transcriptional repressors. Analysis presence GCC box promoter element, thought be responsible ERF binding activity, genes differentially regulated A17 suggests indirect or alternative mechanisms gene may contributing large scale adaptation following infection. Comparison profile with that infection symbiotic bacterium Sinorhizobium medicae legumes adapted perform diverse roles balance against pathogens symbiosis beneficial microorganisms same root tissue.