Osteopontin regulates dentin and alveolar bone development and mineralization

摘要: Abstract The periodontal complex is essential for tooth attachment and function includes the mineralized tissues, cementum alveolar bone, separated by unmineralized ligament (PDL). To gain insights into factors regulating cementum-PDL bone-PDL borders protecting against ectopic calcification within PDL, we employed a proteomic approach to analyze PDL tissue from progressive ankylosis knock-out ( Ank −/− ) mice, featuring reduced PP i , rapid cementogenesis, excessive acellular cementum. Using this approach, identified matrix protein osteopontin Spp1 /OPN) as an elevated factor of interest in mouse molar PDL. We studied role OPN dental development function. During wild-type (WT) mRNA was transiently expressed cementoblasts strongly bone osteoblasts. Developmental analysis 14 240 days postnatal (dpn) indicated normal histological structures comparable WT control mice. Microcomputed tomography (micro-CT) at 30 90 dpn revealed significantly increased volumes mineral densities dentin while pulp were decreased increased. However, growth unaltered Quantitative PCR periodontal-derived failed identify potential local compensators influencing vs. mice 26 dpn. genetically deleted on background determine whether /OPN tissues when space challenged hypercementosis ; double deficient did not exhibit greater than that Based these data, conclude has non-redundant formation mineralization influences properties pulp, but does apposition. These findings may inform therapies targeted controlling soft calcification.