Characterization and immobilization of protease and lipase on chitin-starch material as a novel matrix
作者： Wesen Adel Mehdi , Atheer Awad Mehde , Mahmut Özacar , Zeynep Özacar
DOI: 10.1016/J.IJBIOMAC.2018.04.195
关键词:
摘要: Abstract Protease and lipase were purified from sunflower seeds by frequent purification steps with molecular weights of 72.90 kDa 27.50 kDa, respectively. The protease immobilized on various carriers different methods immobilization including physical adsorption, ionic binding covalent binding. enzymes prepared a new support materials made via the combination chitin starch had highest activates. was carried out in simpler way compared other which require chemicals complicated procedures is difficult expensive. influence reusability, pH, thermal storage stability immobilizing to free enzyme studied. chitin + starch used catalyze reactions through enhanced washing process. After adding starch, stain removal percentage detergents 78% 84%, We approve that these novel present approach synthesis multi biocatalysts cheap friendly environmental sources.
sci-hub.se 参考文章(49)
Laura M. Radonic, Dalia M. Lewi, Nilda E. López, H. Esteban Hopp, Alejandro S. Escandón, Marisa López Bilbao, Sunflower ( Helianthus annuus L.) Methods of Molecular Biology. ,vol. 1224, pp. 47- 55 ,(2006) , 10.1007/978-1-4939-1658-0_5
Jeffrey B. Harborne, Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis ,(1973)
Alexander M. Klibanov, Improving enzymes by using them in organic solvents Nature. ,vol. 409, pp. 241- 246 ,(2001) , 10.1038/35051719
Da-You Xu, Yang Yang, Zhen Yang, Activity and stability of cross-linked tyrosinase aggregates in aqueous and nonaqueous media Journal of Biotechnology. ,vol. 152, pp. 30- 36 ,(2011) , 10.1016/J.JBIOTEC.2011.01.014
Fakhreia A. Al-Sagheer, Enas I. Ibrahim, Khaled D. Khalil, Crystallinity, antimicrobial activity and dyeing properties of chitosan-g-poly(N-acryloyl morpholine) copolymer European Polymer Journal. ,vol. 58, pp. 164- 172 ,(2014) , 10.1016/J.EURPOLYMJ.2014.06.027
Carrie Cupp-Enyard, Sigma's Non-specific Protease Activity Assay - Casein as a Substrate Journal of Visualized Experiments. pp. 899- ,(2008) , 10.3791/899
K.M. Renuka Prasad, Tumkur K. Virupaksha, Purification and characterization of a protease from jackfruit latex Phytochemistry. ,vol. 29, pp. 1763- 1766 ,(1990) , 10.1016/0031-9422(90)85011-4
Mengfan Wang, Wei Qi, Chenxi Jia, Yufei Ren, Rongxin Su, Zhimin He, Enhancement of activity of cross-linked enzyme aggregates by a sugar-assisted precipitation strategy: Technical development and molecular mechanism Journal of Biotechnology. ,vol. 156, pp. 30- 38 ,(2011) , 10.1016/J.JBIOTEC.2011.08.002
Luciana Maria Fedatto, Maria Estela Silva-Stenico, Augusto Etchegaray, Flávia TH Pacheco, Jorge LM Rodrigues, Siu Mui Tsai, None, Detection and characterization of protease secreted by the plant pathogen Xylella fastidiosa Microbiological Research. ,vol. 161, pp. 263- 272 ,(2006) , 10.1016/J.MICRES.2005.10.001
Ritu Tripathi, Jyoti Singh, Randhir kumar Bharti, Indu Shekhar Thakur, Isolation, Purification and Characterization of Lipase from Microbacterium sp. and its Application in Biodiesel Production☆ Energy Procedia. ,vol. 54, pp. 518- 529 ,(2014) , 10.1016/J.EGYPRO.2014.07.293