Prucalopride Inhibits Proliferation of Ovarian Cancer Cells via Phosphatidylinositol 3-Kinase (PI3K) Signaling Pathway.

摘要: BACKGROUND Ovarian cancer is the second most common malignant tumor of female reproductive system and leading cause death gynecological malignancies, but at present there no effective safe therapy. There previously published report on anti-cancer effect prucalopride, which a high-affinity 5-HT4 receptor. The aim study was to determine whether prucalopride can inhibit proliferation ovarian cells. MATERIAL AND METHODS cell viability detected by use Cell Counting Kit-8 (CCK-8) assay. invasion migration SKOV3 OVCAR3 cells Transwell apoptosis flow detection Caspase-Glo 3/7 Assay Systems. apoptosis-related proteins, autophagy marker related-factors phosphatidylinositol 3-kinase (PI3K) were Western blot. RESULTS CCK-8 test showed that inhibited growth lines OVCAR3. In assay, migration. Furthermore, we found expression anti-apoptotic protein Bcl-2 decreased, whereas pro-apoptotic Caspase3 Bax increased in line treated with as well cleaved PARP. addition, p-AKT, p-mTOR, p70S6K decreased prucalopride-treated group, LC3-II/I Beclin1 significantly increased, p62 decreased. CONCLUSIONS reveals cells, inhibits proliferation, migration, invasion, induces autophagy, may be regulated PI3K signaling pathway. These results suggest has potential new drug for clinical treatment.