Intracerebral injection of human mesenchymal stem cells impacts cerebral microvasculature after experimental stroke: MRI study

摘要: Stroke, the leading cause of disability, lacks treatment beyond thrombolysis. The acute injection human mesenchymal stem cells (hMSCs) provides a benefit which could be mediated by an enhancement angiogenesis. A clinical autologous graft requires hMSC culture delay incompatible with administration. This study evaluates cerebral microvascular changes after delayed hMSCs. At day 8 middle artery occlusion (MCAo), two groups rats received intracerebral in damaged brain either 10 μL cell suspension medium (MCAo-PBS, n = 4) or 4 × 10(5) hMSCs (MCAo-hMSC, n = 5). Two control healthy underwent same procedures right hemisphere (control-PBS, n = 6; control-hMSC, effect on microvasculature was assessed MRI using three parameters: apparent diffusion coefficient (ADC), blood volume (CBV) and vessel size index (VSI). 9, eight additional were euthanised for histological parameters (CBV, VSI vascular fraction). No ADC difference observed between MCAo groups. One injection, abolished CBV increase lesion (MCAo-hMSC: 1.7 ± 0.1% versus MCAo-PBS: 2.2 ± 0.2%) (vasodilation) secondary to ischaemia. Histological analysis at 9 confirmed that modified fraction) lesion. ADC, differences stroke post-acute phase, rapidly transiently modifies microvasculature. can monitored vivo MRI. Copyright © 2012 John Wiley & Sons, Ltd.