Microbiological and clinical evaluation of the isolator lysis-centrifugation blood culture tube.

摘要: In a controlled evaluation of 6,010 blood cultures, the yield clinically significant microorganisms was greater from lysis-centrifugation system (Isolator, Du Pont Co.) than nonvented vacuum bottle containing tryptic soy broth with sodium polyanetholesulfonate and CO2 vented biphasic brain heart infusion medium polyanetholesulfonate. The Isolator significantly increased frequency isolation Staphylococcus aureus Candida spp. decreased time required for detection S. aureus, Pseudomonas aeruginosa, spp.; however, anaerobic bacteria were recovered more frequently bottles broth, pneumococci both systems. Contamination cultures either system. Regardless number obtained per septic episode, detected microbiologically proven bacteremia or fungemia in patients detection.