Inhibition of West Nile virus entry by using a recombinant domain III from the envelope glycoprotein.
作者： J. J. H. Chu , R. Rajamanonmani , J. Li , R. Bhuvanakantham , J. Lescar
关键词:
摘要: The envelope glycoprotein located at the outermost surface of flavivirus particle mediates entry virus into host cells. In this study, involvement domain III West Nile (WNV-DIII) protein in binding to cell was investigated. WNV-DIII first expressed as a recombinant and purified after solubilization refolding procedure. refolded displays content β-sheets consistent with known homologous structures other DIII, shown by using circular dichroism analysis. Purified able inhibit WNV Vero cells C6/36 mosquito Recombinant only partially blocked dengue-2 (Den 2) However, Den 2 effectively WNV-DIII. Murine polyclonal serum produced against inhibited infection much lesser extent virus, demonstrated plaque neutralization assays. Together these results provided strong evidence that immunoglobulin-like DIII is responsible for receptor on data also suggest similar attachment molecule(s) or receptor(s) were used
sgmjournals.org参考文章(36)
John T. Roehrig, Antigenic structure of flavivirus proteins. Advances in Virus Research. ,vol. 59, pp. 141- 175 ,(2003) , 10.1016/S0065-3527(03)59005-4
G Wengler, G Wengler, Cell-associated West Nile flavivirus is covered with E+pre-M protein heterodimers which are destroyed and reorganized by proteolytic cleavage during virus release. Journal of Virology. ,vol. 63, pp. 2521- 2526 ,(1989) , 10.1128/JVI.63.6.2521-2526.1989
Franz X Heinz, Steven L Allison, Flavivirus structure and membrane fusion. Advances in Virus Research. ,vol. 59, pp. 63- 97 ,(2003) , 10.1016/S0065-3527(03)59003-0
Richard J Kuhn, Wei Zhang, Michael G Rossmann, Sergei V Pletnev, Jeroen Corver, Edith Lenches, Christopher T Jones, Suchetana Mukhopadhyay, Paul R Chipman, Ellen G Strauss, Timothy S Baker, James H Strauss, None, Structure of dengue virus: implications for flavivirus organization, maturation, and fusion. Cell. ,vol. 108, pp. 717- 725 ,(2002) , 10.1016/S0092-8674(02)00660-8
Robbert G. van der Most, Jeroen Corver, James H. Strauss, Mutagenesis of the RGD motif in the yellow fever virus 17D envelope protein. Virology. ,vol. 265, pp. 83- 95 ,(1999) , 10.1006/VIRO.1999.0026
J.J.H Chu, M.L Ng, Characterization of a 105-kDa plasma membrane associated glycoprotein that is involved in West Nile virus binding and infection. Virology. ,vol. 312, pp. 458- 469 ,(2003) , 10.1016/S0042-6822(03)00261-7
Fouad N. Boctor, Charles H. Calisher, James B. Peter, Dot-ELISA for serodiagnosis of human infections due to Western equine encephalitis virus☆ Journal of Virological Methods. ,vol. 26, pp. 305- 311 ,(1989) , 10.1016/0166-0934(89)90112-2
Yaping Chen, Terry Maguire, Ronald E. Hileman, Jonathan R. Fromm, Jeffrey D. Esko, Robert J. Linhardt, Rory M. Marks, Dengue virus infectivity depends on envelope protein binding to target cell heparan sulfate Nature Medicine. ,vol. 3, pp. 866- 871 ,(1997) , 10.1038/NM0897-866
Narasimha Sreerama, Robert W. Woody, Estimation of Protein Secondary Structure from Circular Dichroism Spectra: Comparison of CONTIN, SELCON, and CDSSTR Methods with an Expanded Reference Set Analytical Biochemistry. ,vol. 287, pp. 252- 260 ,(2000) , 10.1006/ABIO.2000.4880
Helga Kroschewski, Steven L Allison, Franz X Heinz, Christian W Mandl, Role of heparan sulfate for attachment and entry of tick-borne encephalitis virus. Virology. ,vol. 308, pp. 92- 100 ,(2003) , 10.1016/S0042-6822(02)00097-1