Inhibition of Mast Cell Degranulation Relieves Visceral Hypersensitivity Induced by Pancreatic Carcinoma in Mice.

摘要: Cancer pain induced by pancreatic carcinoma is one of the most common symptoms and difficult to endure, especially in advanced stage. Evidence suggests that mast cells are recruited degranulate enteric disease-related visceral hypersensitivity. However, whether promote remains unclear. Here, using toluidine blue staining western blotting, we observed were dramatically tissues surrounding carcinoma, but not inside patients with severe pain. The levels cell degranulation products, including tryptase, histamine, nerve growth factor, significantly increased pericarcinoma relative their normal controls, as evidenced enzyme-linked immunosorbent assay. We determined systemic administration secretagogue compound 48/80 exacerbated carcinoma-induced hypersensitivity a male BALB/c nude mouse model assessed measuring hunching behavior scores mechanical withdrawal response frequency evoked von Frey stimulation. In contrast, stabilizer ketotifen dose-dependently alleviated cancer addition, incomplete development abdominal hyperalgesia cell-deficient mice carcinoma. did further attenuate Finally, confirmed intraplantar injection supernatants from caused acute somatic nociception. conclusion, our findings suggest contribute through enrichment tissues. inhibition may be potential strategy for therapeutic treatment chronic