Hyaluronan scaffold supports osteogenic differentiation of bone marrow concentrate cells.

摘要: Osteochondral lesions are considered a challenge for orthopedic surgeons. Currently, the treatments available often unsatisfactory and unable to stimulate tissue regeneration. Tissue engineering offers new therapeutic strategy, taking into account role exerted by cells, biomaterial growth factors in restoring damage. In this light, Mesenchymal Stem Cells (MSCs) have been indicated as fascinating tool regenerative medicine thanks their ability differentiate bone, cartilage adipose tissue. However, vitro-cultivation of MSCs could be associated with some risks such de-differentiation/reprogramming, infection contaminations cells. To overcome these shortcomings, approach is represented use Bone Marrow Concentrate (BMC), that allow delivery cells surrounded microenvironment injured For purpose, require tridimensional scaffold can support adhesion, proliferation differentiation. This study focused on potentiality BMC seeded onto hyaluronan-based (Hyaff-11) osteogenic lineage. process depends specific interaction between derived from bone marrow (surrounded niche) scaffold, create an environment able regeneration damaged The data obtained present demonstrate grown Hyaff-11 toward sense, producing genes matrix proteins.