A standard photomap of the ovarian nurse cell chromosomes for the dominant malaria vector in Europe and Middle East Anopheles sacharovi.

摘要: Anopheles sacharovi is a dominant malaria vector species in South Europe and the Middle East which has highly plastic behaviour at both adult larval stages. Such plasticity prevented this from eradication by several anti-vector campaigns. The development of new genome-based strategies for control will benefit genome sequencing physical chromosome mapping mosquito. Although cytogenetic photomap chromosomes salivary glands An. been developed, no map suitable available. Mosquitoes study were collected stage animal shelters Armenia. Polytene preparations prepared ovarian nurse cells. Fluorescent situ hybridization (FISH) was performed using PCR amplified probes. This constructed high-quality standard polytene cells sacharovi. Following previous nomenclature, sub-divided into 39 numbered 119 lettered sub-divisions. Chromosomal landmarks recognition described. Using FISH, 4 PCR-amplified genic probes mapped to chromosomes. positions demonstrated gene order reshuffling between atroparvus not seen cytologically. In addition, described specific chromosomal that can be used cytotaxonomic diagnostics based on banding pattern its cell validated utility mapping. Based map, features identification have assist creating chromosome-based assembly mosquito developing tools other Maculipennis group.