Temporal Regulation of Herpes Simplex Virus Type 1UL24mRNA Expression via Differential Polyadenylation

摘要: Abstract Using Northern blot, primer extension, and S1 nuclease analyses of wild-type deletion-containing herpes simplex type 1 viruses, we found that UL24 sequences are contained in six different transcripts originate from three previously identified mRNA start sites. Thus, the represent pairs 5′ coterminal mRNAs. Each transcript pair consists a short species whose 3′ end corresponds to polyadenylation signal located just downstream open reading frame, longer UL26 gene. Maximal accumulation was at early times during infection, while did not decrease late times. Consistent with kinetics, were less sensitive drugs inhibited viral DNA replication than which exhibited leaky–late kinetics. Quantitative analysis indicated ends corresponding site significantly more abundant differential determines kinetics transcripts.