Preparation and physicochemical properties of digested collagen fragments with varying molecular weights

摘要: The porcine dermal collagen molecules were successfully digested by clostridium histolyticun collagenases (CHC) and then ultra-filtrated using varying grades of ultra-filtration membranes. Gel permeation analyses revealed that fragments with molecular weights segregated membranes pore sizes. Fourier transform infra-red suggest prepared after still preserve certain percentages triple helix structures molecules, although the preserved present in reduce significantly as their Mw values reduce. Thermal denaturation temperature analysis thermal degradation decrease absorbed/desorbed amounts PA6/PP flocking specimens are higher than those original collagens, increase Possible reasons accounting for above degradation, physicochemical, absorbing desorbing properties original, reported.