Controlled nucleation of hydroxyapatite on alginate scaffolds for stem cell-based bone tissue engineering

摘要: Current bone tissue engineering strategies aim to grow a similar native by combining cells and biologically active molecules with scaffold material. In this study, macroporous made from the seaweed- derived polymer alginate was synthesized mineralized for cell-based applications. Nuclea- tion of bone-like hydroxyapatite mineral achieved incubating in modified simulated body fluids (mSBF) 4 weeks. Analysis using scanning electron micros- copy energy dispersive x-ray analysis indicated growth continuous layer primarily composed cal- cium phosphorous. X-ray diffraction showed peaks associated hydroxyapatite, major inorganic constituent human tissue. addition characterization, ability control nucleation on sur- face, into bulk material, or inner pore surfa- ces scaffolds demonstrated. Finally, MSCs attached proliferated cell attachment improved when seeding coated scaffolds. This novel alginate- HAP composite material could be used as scaf- fold deliver cells, perhaps also molecules. V C