Microinjection of arginase into enzyme-deficient cells with the isolated glycoproteins of Sendai virus as fusogen.

摘要: A method of introducing enzymes into the cytoplasm fibroblasts in culture is described. Erythrocytes obtained from normal and arginase-deficient individuals were loaded with arginase vitro fused to mouse human fibroblasts. Erythrocyte ghost-fibroblast fusion was quantified by a 14C-radioactive assay for solubilized Fusion successfully induced Sendai virus also isolated glycoproteins virus. After activity associated Fibroblasts 700--1500 U arginase/mg cell protein; this enzyme 5- 10-times higher than that normally found The enrichment indicated between four ten ghosts had per fibroblast. use viral proteins mediate transfer cells vivo might alleviate clinical complications inherent whole virions. replacement technique described report hyperargininemic model system should be applicable group inborn errors metabolism characterized deficiency an localized cytoplasmic compartment cells.