Mycobacterium tuberculosis strains exhibit differential and strain-specific molecular signatures in pulmonary epithelial cells.

摘要: Although pulmonary epithelial cells are integral to innate and adaptive immune responses during Mycobacterium tuberculosis infection, global transcriptomic changes in these remain largely unknown. Changes gene expression induced infected with M. tuberculosis F15/LAM4/KZN, F11, F28, Beijing Unique genotypes were investigated by RNA sequencing (RNA-Seq). The Illumina HiSeq 2000 platform generated 50 bp reads that mapped the human genome (Hg19) using Tophat (2.0.10). Differential different strains relative uninfected was quantified compared Cufflinks (2.1.0) MeV (4.0.9), respectively. Gene varied among total number of genes as follows: F15/LAM4/KZN (1187), (1252), F11 (1639), F28 (870), (886) H37Rv (1179). A subset 292 commonly all strains, where 52 down-regulated while 240 up-regulated. Differentially expressed strain-specific signatures (138), (52), (255), (55), (186) (125). Strain-specific molecular associated functional pathways observed only for certain biological functions may be other strain signatures. This study demonstrated induce differential cells. Specific clinical can further explored novel host-associated biomarkers adjunctive immunotherapies.