Multifunctional Transmembrane Protein Ligands for Cell-Specific Targeting of Plasma Membrane-Derived Vesicles.

摘要: Liposomes and nanoparticles that bind selectively to cell-surface receptors can target specific populations of cells. However, chemical conjugation ligands these particles is difficult control, frequently limiting ligand uniformity complexity. In contrast, the surfaces living cells are decorated with highly uniform sophisticated transmembrane proteins. Toward harnessing cellular capabilities, here it demonstrated plasma membrane vesicles (PMVs) derived from donor display engineered protein precisely on basis receptor expression. These multifunctional targeting proteins incorporate (i) a ligand, (ii) an intrinsically disordered spacer make sterically accessible, (iii) fluorescent domain enables quantification density PMV surface. PMVs affinity for epidermal growth factor (EGFR) at increasing concentrations breast cancer express levels EGFR. Further, as example generality this approach, expressing single-domain antibody against green fluorescence (eGFP) eGFP-tagged selectivity ≈50:1. The results demonstrate versatility cell systems, suggesting diverse applications drug delivery tissue engineering.