Fibrous Hydrogels for Cell Encapsulation: A Modular and Supramolecular Approach

摘要: Artificial 3-dimensional (3D) cell culture systems, which mimic the extracellular matrix (ECM), hold great potential as models to study cellular processes under controlled conditions. The natural ECM is a 3D structure composed of fibrous hydrogel that provides both mechanical and biochemical cues instruct behavior. Here we present an ECM-mimicking genetically engineered protein-based system combines several key features: (1) Mild straightforward encapsulation meters ease ut I am not so sure.encapsulation cells, without need external crosslinker. (2) Supramolecular assembly resulting in architecture recapitulates some unique characteristics ECM, i.e. strain-stiffening self-healing (3) A modular approach allowing incorporation cue density (integrin binding RGD domains). We tested gels by encapsulating MG-63 osteoblastic cells found encapsulated only respond higher density, but also overall gel concentration. Cells 1% 2% (weight fraction) protein showed spreading proliferation, provided relative at least 50%. In contrast, 4% very little proliferation occurred, even for 100%. independent control over obtained this renders our hydrogels suitable responses highly defined