Enzyme Engineering for In Situ Immobilization.
作者： Fabian Rehm , Shuxiong Chen , Bernd Rehm
DOI: 10.3390/MOLECULES21101370
关键词:
摘要: Enzymes are used as biocatalysts in a vast range of industrial applications. Immobilization enzymes to solid supports or their self-assembly into insoluble particles enhances applicability by strongly improving properties such stability changing environments, re-usability and continuous biocatalytic processes. The possibility co-immobilizing various functionally related involved multistep synthesis, conversion degradation reactions enables the design multifunctional biocatalyst with enhanced performance compared soluble counterparts. This review provides brief overview up-to-date vitro immobilization strategies while focusing on recent advances enzyme engineering towards situ particles. In approaches include bioengineering bacteria abundantly form enzymatically active inclusion bodies inclusions enzyme-coated polyhydroxyalkanoate granules. These one-step production for immobilized avoid prefabrication carrier well chemical cross-linking attachment support material controlled oriented display fraction accessible catalytic sites hence functional enzymes.
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