Molecular characterization of branchial aquaporin 1aa and effects of seawater acclimation, emersion or ammonia exposure on its mRNA expression in the gills, gut, kidney and skin of the freshwater climbing perch, Anabas testudineus.

摘要: We obtained a full cDNA coding sequence of aquaporin 1aa (aqp1aa) from the gills freshwater climbing perch, Anabas testudineus, which had highest expression in and skin, suggesting an important role Aqp1aa these organs. Since seawater acclimation no significant effects on branchial intestinal aqp1aa mRNA expression, since gut was extremely low, it can be deduced that Aqp1aa, despite being water channel, did not play osmoregulatory A. testudineus. However, terrestrial exposure led to increases skin would lead evaporative loss, results further support proposition function predominantly for permeation through skin. Rather, increased might necessary facilitate ammonia excretion during emersion, because testudineus is known utilize amino acids as energy sources locomotor activity with production land. Furthermore, resulted decreases presumably reduce influx loading. This corroborates previous reports AQP1 able permeation. molecular characterization revealed its intrinsic aquapore NH3 transport. Hence, probably permeated central fifth pore tetramer suggested previously. Taken together, our indicate have greater physiological than osmoregulation