Protein tyrosine phosphatase SHP2 promotes invadopodia formation through suppression of Rho signaling.

摘要: Invadopodia are actin-enriched membrane protrusions that important for extracellular matrix degradation and invasive cell motility. Src homolog domain-containing phosphatase 2 (SHP2), a non-receptor protein tyrosine phosphatase, has been shown to play an role in promoting cancer metastasis, but the underlying mechanism is unclear. In this study, we found depletion of SHP2 by short-hairpin RNA suppressed invadopodia formation several lines, particularly SAS head neck squamous line. contrast, overexpression promoted CAL27 line, which expresses low levels endogenous SHP2. The cells significantly decreased their suppression was restored Clostridium botulinum C3 exoenzyme (a Rho GTPase inhibitor) or Y27632 specific inhibitor Rho-associated kinase). Together, our results suggest may promote through inhibition signaling cells.