Metallization and investigation of electrical properties of in vitro recrystallized mSbsC-eGFP assemblies.
作者： Nuriye Korkmaz , Felix Börrnert , Denny Köhler , Rafael G Mendes , Alicja Bachmatiuk
DOI: 10.1088/0957-4484/22/37/375606
关键词:
摘要: Surface layer (SL) proteins are self-assembling nanosized arrays which can be recrystallized in solution or on surfaces. In this paper, we investigate the metallization, contact potential difference and conductivity of in?vitro mSbsC-eGFP tube-like assemblies for possible applications nanobiotechnology. Treatment structures with 150?mM Pt salt resulted formation metallized SL decorated nanoparticles (? > 3?nm) were closely packed aggregated into metal clusters. Kelvin probe force microscopy (KPFM) measurements revealed that unmetallized templates showed different surface behaviours, demonstrating coating changes electrostatic characteristics assemblies. In?situ not conductive. Metallized samples linear I?V dependence between ? 1 + 1?V a ~ 103?S?m 1.
harvard.edu
sci-hub.se 参考文章(38)
Uwe B Sleytr, Eva M Egelseer, Nicola Ilk, Dietmar Pum, Bernhard Schuster, S-Layers as a basic building block in a molecular construction kit. FEBS Journal. ,vol. 274, pp. 323- 334 ,(2007) , 10.1111/J.1742-4658.2006.05606.X
S Schultze-Lam, G Harauz, T J Beveridge, Participation of a cyanobacterial S layer in fine-grain mineral formation. Journal of Bacteriology. ,vol. 174, pp. 7971- 7981 ,(1992) , 10.1128/JB.174.24.7971-7981.1992
Hans Sørensen, Kim Mortensen, Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli Microbial Cell Factories. ,vol. 4, pp. 1- 8 ,(2005) , 10.1186/1475-2859-4-1
N. Ilk, E.M. Egelseer, J. Ferner-Ortner, S. Küpcü, D. Pum, B. Schuster, U.B. Sleytr, Surfaces functionalized with self-assembling S-layer fusion proteins for nanobiotechnological applications Colloids and Surfaces A: Physicochemical and Engineering Aspects. ,vol. 321, pp. 163- 167 ,(2008) , 10.1016/J.COLSURFA.2007.12.038
Mohamed L Merroun, Johannes Raff, André Rossberg, Christoph Hennig, Tobias Reich, Sonja Selenska-Pobell, None, Complexation of Uranium by Cells and S-Layer Sheets of Bacillus sphaericus JG-A12 Applied and Environmental Microbiology. ,vol. 71, pp. 5532- 5543 ,(2005) , 10.1128/AEM.71.9.5532-5543.2005
Billel Salhi, François Vaurette, Bruno Grandidier, Didier Stiévenard, Oleg Melnyk, Yannick Coffinier, Rabah Boukherroub, The collagen assisted self-assembly of silicon nanowires. Nanotechnology. ,vol. 20, pp. 235601- ,(2009) , 10.1088/0957-4484/20/23/235601
Tae-Hwan Kim, John F Wendelken, An-Ping Li, Gaohui Du, Wenzhi Li, Probing electrical transport in individual carbon nanotubes and junctions. Nanotechnology. ,vol. 19, pp. 485201- 485201 ,(2008) , 10.1088/0957-4484/19/48/485201
Jan Šmarda, David Šmajs, Jiřı́ Komrska, Vladislav Krzyžánek, S-layers on cell walls of cyanobacteria Micron. ,vol. 33, pp. 257- 277 ,(2002) , 10.1016/S0968-4328(01)00031-2
Uwe B Sleytr, Terry J Beveridge, Bacterial S-layers Trends in Microbiology. ,vol. 7, pp. 253- 260 ,(1999) , 10.1016/S0966-842X(99)01513-9
Carl Leung, Helen Kinns, Bart W. Hoogenboom, Stefan Howorka, Patrick Mesquida, Imaging surface charges of individual biomolecules. Nano Letters. ,vol. 9, pp. 2769- 2773 ,(2009) , 10.1021/NL9012979