[3] Immobilization of enzymes to agar, agarose, and sephadex support

摘要: Publisher Summary This chapter discusses the immobilization of enzymes to agar, agarose, and Sephadex supports. From a purely economic point view, cellulose starch are perhaps most attractive starting materials for immobilization. Their chemistry is well understood. In spite these facts, they have certain disadvantages that make them less suitable granular immobilized enzymes, serious which an improper macroporous structure susceptibility microbial disintegration. Dextran in cross-linked form, Sephadex, under conditions superior starch. Enzymes fixed exhibit higher relative activity than corresponding cellulose-bound enzymes. Presumably, microenvironment within gels disruptive exertion catalytic action fibrous with its microcrystalline regions. Starch more easily attacked by microorganisms Sephadex. Agarose has been found be very suited as matrix production biospecific adsorbents, although extensively purified agar may better choice reasons technical applications.