13C NMR for the assessment of human brain glucose metabolism in vivo.
作者： Nicolau Beckmann , Igor Turkalj , Joachim Seelig , Ulrich Keller
DOI: 10.1021/BI00240A002
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摘要: Proton-decoupled 13C NMR spectra of the human head were obtained during hyperglycemic glucose clamping using intravenous infusions [1-13C]glucose in normal volunteers. In addition to signals mobile lipids, a variety new metabolite resonances could be resolved for first time brain. At an enrichment level 20% [1-13C]glucose, alpha- and beta-glucose at 92.7 96.6 ppm, respectively, detected brain after only infusion period 15 min. The spatial localization different regions interest was confirmed by spectroscopic imaging with resolution 9 Increasing 99% not improved but allowed detection metabolic breakdown products [1-13C]glucose. course label incorporation into C2, C3, C4 glutamate/glutamine lactate recorded These results suggest possibility obtaining time-resolved, spatially selective, chemically specific information on body.
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