Use of the local lymph node assay for the estimation of relative contact allergenic potency

摘要: The effective toxicological evaluation of skin sensitization demands that potential contact allergens are identified and the likely risks among exposed populations assessed. By definition, chemicals which possess property potentially capable causing allergic dermatitis (ACD) in humans. However, this hazard is not an all-or-none phenomenon; clear dose-response relationships can be discerned thresholds for both induction elicitation dermatitis. Commonly, these parameters grouped under heading potency, determination vital risk assessment. In present investigation, local lymph node assay (LLNA) has been employed to determine relative potency a range 20 chemicals. parameter used estimated concentration required produce 3-fold increase draining lymph-node cell proliferative activity, EC3 value. These measurements have compared with assessment human sensitizing selected chemicals, each being assigned 1 5 classes based on their potency. value, derived from LLNA work carried out acetone/ olive oil vehicle, correlated well classification, strongest sensitizers having low values (