Chapter 5. In vivo analysis of the decay of transcripts generated by cytoplasmic RNA viruses.

摘要: Abstract The field of RNA decay has grown extensively over the last few years and numerous pathways have been identified characterized. This is a truly powerful machinery for both regulation quality control gene expression. It very likely that transcripts viruses must successfully confront this arsenal enzymes binding factors in order to establish productive infection. interface an understudied branch virology needs be explored if we are fully comprehend molecular biology virus–cell interactions. Research area potential increase our understanding fundamentals mRNA stability viral biology, perhaps leading novel antiviral approaches. chapter discusses methods examining half-lives RNAs during natural infection, including purification subsequent analysis deadenylation decay. Additionally, hybrid selection protocol identifying viral-specific small generated infection by RNAi cellular described.