A synergistic combination of diatomaceous earth with Au nanoparticles as a periodically ordered, button-like substrate for SERS analysis of the chemical composition of eccrine sweat in latent fingerprints

摘要: The artistry and the amazingly beautiful hierarchical three-dimensional (3D) patterning of diatoms make them ideal biological scaffolds. Here, fraction-free ultra-high-purity diatomaceous earth has been successfully prepared by low speed centrifugation in saturated sucrose solution calcination at 600 °C for 2 h. citrate-stabilized 18 ± 2.4 32 5.4 nm Au nanoparticles were singly, uniformly densely self-assembled onto amino-functionalized templates following their surface morphologies via electrostatic interactions, during which control experiment appropriate parameter exploration performed. formed nanoparticle coated fine-grained composite materials pressed into hard, button-like tablets with a fixed diameter 13 mm thickness mm, then two fabricated types portable used surface-enhanced Raman spectroscopy (SERS) detection, enhancement factors (EFs), SERS reproducibility long-term stability testified using rhodamine 6G (R6G) as probe molecule. tablet composed earth-templated arrays had higher EF was applied to analysis trace chemical composition eccrine sweat latent fingerprints. It may reveal medical condition an individual analyzing obtained spectra, suggesting that SERS-based methodology, due its high sensitivity, potentially wide range applications fields need such diagnostics forensic investigations. Moreover, button-like, 3D periodically ordered substrate synergistic combination this work is robust, easy carry around, applicable on-site detection.