EdU statt BrdU: eine Alternative zur Detektion der Zellproliferation
作者： Birgit Oberleitner , Antonio Manetto , Thomas Frischmuth
DOI: 10.1007/S12268-014-0428-Z
关键词:
摘要: The use of BrdU (5′-bromo-2′-deoxyuridine) is a widely accepted method for the detection cell proliferation. Despite good performance, this has yet shown some disadvantages such as destruction target DNA in order to allow antibody-driven colorimetric/fluorescent newly synthesized cells. This harsh destructing attack on system can be overcome by using EdU (5′-ethynyl-2′-deoxyuridine) innovative based click chemistry.
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