Impact of Temperature, Nutrients and Heavy Metals on Bacterial Diversity and Ecosystem Functioning Studied by Freshwater Microcosms and High-Throughput DNA Sequencing.
作者： Yuan , T. M. , Zhang , H. H. , Feng
DOI: 10.1007/S00284-020-02138-5
关键词:
摘要: Microbial communities are fundamental components in freshwater, and community shifts ecosystem structure indicative of changing environmental conditions. This study aimed at investigating the influence key parameters on bacterial diversity functioning (i.e. organic matter breakdown) laboratory freshwater microcosms. The effects varying temperatures (5, 20 35 °C), nutrients (representing low, medium high urbanization) heavy metals Copper (Cu) Zinc (Zn) (OM) breakdown were studied by using leaf bags capsules filled with polycaprolactonediol-2000 (PCP-2000), respectively. leaf-associated was determined next-generation sequencing SSU rRNA gene amplicons. results showed that increased temperature (35 °C) more operational taxonomic units (OTUs) as compared to (20 °C) or low (5 °C) temperatures, whereas nutrient variation had fewer structure. In contrast, presence metals, especially concentrations (100 μM) Cu, reduced number OTUs community. higher levels accelerated PCP-2000 rate, but this impeded a concentration Cu short term, though no effect Zn rate observed. overall indicate variated among factors affect systems.
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