Efficient KEX2-Like Processing of a Glucoamylase-Interleukin-6 Fusion Protein by Aspergillus Nidulans and Secretion of Mature Interleukin-6
作者： Roland Contreras , Dirk Carrez , James R. Kinghorn , Cees A. M. J. J. van den Hondel , Walter Fiers
DOI: 10.1038/NBT0491-378
关键词:
摘要: We have designed an expression vector for the secretion of human interleukin-6 (hIL-6) in which mature protein is fused through a spacer peptide, containing KEX-2 like processing signal, to entire Aspergillus niger glucoamylase (glaA) gene. Transformation nidulans with this results fungal strains secreting equimolar amounts and IL-6 proteins. The KEX2-type Lys-Arg, recognized cleaved efficiently by enzyme present A. resulting authentic hIL-6 at levels up 5 mg/l.
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