Identification of cleavage sites in the HIV-1 TAR RNA by 10-23 and 8-17 catalytic motif containing DNA enzymes

摘要: A quick identification of a cleavage site in the target RNA molecule to obtain sequence-specific by either catalytic (ribozymes) or DNA (DNA enzymes) is very important for achieving gene-specific suppression. These molecules could also provide information on secondary and tertiary structure molecule. We have exploited use two kinds enzymes, namely, 10-23 8-17 motif containing achieve these objectives. identified several enzyme sites human immunodeficiency virus type 1 (HIV-1) transactivation response element (TAR) RNA-a structural feature present at 5' end all HIV-1 transcripts. Most enzymes that cleaved TAR were targeted regions single-stranded predicted structure. Regions be base-paired (stem) failed show any detectable cleavage. The possessing was extremely efficient cleaving full length, as well short, specific efficiency same possessed significantly less comparison, they cleave short molecules, principle, potential down regulate expression transcripts from wide range isolates because this region functionally conserved.