Cytoprotective effect of a bilberry extract against oxidative damage of rat hepatocytes
作者： Kateřina Valentová , Jitka Ulrichová , Ladislav Cvak , Vilím Šimánek
DOI: 10.1016/J.FOODCHEM.2006.02.038
关键词:
摘要: Abstract The effect of a bilberry extract (BE, 25% anthocyanins) against oxidative damage in primary cultures rat hepatocytes, induced by tert -butyl hydroperoxide and allyl alcohol, was investigated. BE displayed cytoprotective effects at 100 500 μg/ml the MTT viability test. It protected cells lactate dehydrogenase leakage lipoperoxidation products formation. Maximum protection (58%) noted using intoxication alcohol. observed is probably due to antioxidant properties its constituents, mainly anthocyanins. scavenged DPPH (IC 50 3.99 ± 0.14 μg/ml) enzymatically generated superoxide radical with an activity equivalent 108 ± 7.2 U dismutase per mg extract. Our results support use extracts functional foods food supplements designed for prevention chronic diseases associated stress.
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sci-hub.se 参考文章(24)
C Deby, G Magotteaux, Relationship between essential fatty acids and tissue antioxidant levels in mice Comptes rendus des séances de la Société de biologie et de ses filiales. ,vol. 164, pp. 2675- 2681 ,(1970)
Michael N. Berry, Isolated Hepatocytes: Preparation, Properties and Applications ,(1991)
John A. Buege, Steven D. Aust, Microsomal lipid peroxidation. Methods in Enzymology. ,vol. 52, pp. 302- 310 ,(1978) , 10.1016/S0076-6879(78)52032-6
Peter Moldéus, Johan Högberg, Sten Orrenius, [4] Isolation and use of liver cells Methods in Enzymology. ,vol. 52, pp. 60- 71 ,(1978) , 10.1016/S0076-6879(78)52006-5
Jitka Psotová, Milan Kolář, Jaromír Soušek, Zdeněk Švagera, Jaroslav Vičar, Jitka Ulrichová, Biological activities of Prunella vulgaris extract Phytotherapy Research. ,vol. 17, pp. 1082- 1087 ,(2003) , 10.1002/PTR.1324
PM Laplaud, A. Lelubre, MJ Chapman, Antioxidant action of Vaccinium myrtillus extract on human low density lipoproteins in vitro: initial observations Fundamental & Clinical Pharmacology. ,vol. 11, pp. 35- 40 ,(1997) , 10.1111/J.1472-8206.1997.TB00166.X
Michel Joyeux, François Mortier, Jacques Fleurentin, Screening of antiradical, antilipoperoxidant and hepatoprotective effects of nine plant extracts used in Caribbean folk medicine Phytotherapy Research. ,vol. 9, pp. 228- 230 ,(1995) , 10.1002/PTR.2650090316
Zdeněk Dvořák, Pavel Kosina, Daniela Walterová, Vilı́m Šimánek, Petr Bachleda, Jitka Ulrichová, Primary cultures of human hepatocytes as a tool in cytotoxicity studies: cell protection against model toxins by flavonolignans obtained from Silybum marianum. Toxicology Letters. ,vol. 137, pp. 201- 212 ,(2003) , 10.1016/S0378-4274(02)00406-X
Patrícia Valentão, Márcia Carvalho, Félix Carvalho, Eduarda Fernandes, Ricardo Pires das Neves, M. Lourdes Pereira, Paula B. Andrade, Rosa M. Seabra, M. Lourdes Bastos, Hypericum androsaemum infusion increases tert-butyl hydroperoxide-induced mice hepatotoxicity in vivo Journal of Ethnopharmacology. ,vol. 94, pp. 345- 351 ,(2004) , 10.1016/J.JEP.2004.06.012
J.-P. Goiffon, M. Brun, M.-J. Bourrier, High-performance liquid chromatography of red fruit anthocyanins Journal of Chromatography A. ,vol. 537, pp. 101- 121 ,(1991) , 10.1016/S0021-9673(01)88890-7