Transplantation of human hepatocytes into tolerized genetically immunocompetent rats
作者： Edwin C Ouyang , Catherine H Wu , Cherie Walton , Kittichai Promrat , George Y Wu
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摘要: AIM: To determine whether normal genetically immunocompetent rodent hosts could be manipulated to accept human hepatocyte transplants with long term survival without immunosuppression. METHODS: Tolerance towards hepatocytes was established by injection of primary or Huh7 hepatoma cells into the peritoneal cavities fetal rats. Corresponding were subsequently transplanted newborn rats via intrasplenic within 24 h after birth. RESULTS: Mixed lymphocyte assays showed that spleen from non-tolerized stimulated proliferate when exposed hepatocytes, while tolerized not. Injections made between 15 d and 17 gestation produced optimal tolerizaton. Transplanted in rat livers visualized immunohistochemical staining albumin. By dot blotting genomic DNA 16 weeks transplantation, it found approximately 2.5 × 105 survived per liver. Human albumin mRNA detected RT-PCR for wk, protein also detectable serum. CONCLUSION: Tolerization an can permit functional hepatocytes.
sci-hub.st 参考文章(22)
Andreas Limmer, Jutta Ohl, Christian Kurts, Hans-Gustaf Ljunggren, Yuval Reiss, Marcus Groettrup, Frank Momburg, Bernd Arnold, Percy A. Knolle, Efficient presentation of exogenous antigen by liver endothelial cells to CD8+ T cells results in antigen-specific T-cell tolerance. Nature Medicine. ,vol. 6, pp. 1348- 1354 ,(2000) , 10.1038/82161
Kazuo Ohashi, Patricia L. Marion, Hiroyuki Nakai, Leonard Meuse, John M. Cullen, Bruno B. Bordier, Ralph Schwall, Harry B. Greenberg, Jeffrey S. Glenn, Mark A. Kay, Sustained survival of human hepatocytes in mice: A model for in vivo infection with human hepatitis B and hepatitis delta viruses Nature Medicine. ,vol. 6, pp. 327- 331 ,(2000) , 10.1038/73187
G.Y. Wu, J.M. Wilson, F. Shalaby, M. Grossman, D.A. Shafritz, C.H. Wu, Receptor-mediated gene delivery in vivo. Partial correction of genetic analbuminemia in Nagase rats. Journal of Biological Chemistry. ,vol. 266, pp. 14338- 14342 ,(1991) , 10.1016/S0021-9258(18)98689-8
John Kiernan, Histological and Histochemical Methods: Theory and Practice ,(1981)
Ronald H. Schwartz, Lula Jackson, William E. Paul, T Lymphocyte-Enriched Murine Peritoneal Exudate Cells I. A Reliable Assay for Antigen-Induced T Lymphocyte Proliferation Journal of Immunology. ,vol. 115, pp. 1330- 1338 ,(1975)
Gary M. Kline, Zhenya Shen, Muhammad Mohiuddin, Vincent Ruggiero, Susan Rostami, Verdi J. DiSesa, Development of tolerance to experimental cardiac allografts in utero The Annals of Thoracic Surgery. ,vol. 57, pp. 72- 75 ,(1994) , 10.1016/0003-4975(94)90367-0
Ehud Ilan, Tatjana Burakova, Shlomo Dagan, Ofer Nussbaum, Ido Lubin, Rachel Eren, Ofer Ben-Moshe, Joseph Arazi, Shoshana Berr, Lewis Neville, Leonard Yuen, Tarek S. Mansour, John Gillard, Ahamed Eid, Oded Jurim, Daniel Shouval, Yair Reisner, Eithan Galun, The hepatitis B virus–trimera mouse: A model for human HBV infection and evaluation of anti-HBV therapeutic agents Hepatology. ,vol. 29, pp. 553- 562 ,(1999) , 10.1002/HEP.510290228
Ginny L. Bumgardner, Jiashun Li, J. David Prologo, Marie Heininger, Charles G. Orosz, Patterns of immune responses evoked by allogeneic hepatocytes: Evidence for independent co-dominant roles for CD4+ and CD8+ T-cell responses in acute rejection Transplantation. ,vol. 68, pp. 555- 562 ,(1999) , 10.1097/00007890-199908270-00019
Jonathan M. Gershoni, George E. Palade, Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter Analytical Biochemistry. ,vol. 124, pp. 396- 405 ,(1982) , 10.1016/0003-2697(82)90056-2
Percy A. Knolle, Guido Gerken, Local control of the immune response in the liver. Immunological Reviews. ,vol. 174, pp. 21- 34 ,(2000) , 10.1034/J.1600-0528.2002.017408.X