The effect of varying fibronectin concentration on the attachment of endothelial cells to polytetrafluoroethylene vascular grafts.

摘要: Endothelial cell seeding onto untreated polytetrafluoroethylene vascular prostheses is inefficient. In an effort to improve attachment, numerous investigators have used fibronectin as a coating material pretreat the luminal surfaces of these prostheses. The concentrations varied enormously, and no one has yet determined most efficient concentration in terms attachment cost. Using endothelial cells labeled with indium 111 oxine we studied effect varying on grafts. Seeding efficiency was significantly better all groups coated grafts, at times (10, 30, 60, 120 minutes), compared uncoated controls (p less than 0.01). Overall, 20 micrograms/ml found be cost since any further increase not accompanied by increased attachment. We now routinely use coat our grafts before seeding.