RANTES- and interleukin-8-induced responses in normal human eosinophils: effects of priming with interleukin-5.

摘要: We report that responses of normal human eosinophils toward the chemokines RANTES and interleukin-8 (IL-8) are modulated upregulated by priming with IL-5. In a modified Boyden chamber assay, we studied migratory members chemokine family RANTES, monocyte chemotactic protein-1 (MCP-1), macrophage inflammatory alpha (MIP-1 alpha) (C-C subfamily), IL-8, platelet factor-4 (PF-4), neutrophil-activating peptide-2 (NAP-2) (C-x-C subfamily). These were also in terms actin polymerization ([Ca2+]i)-mobilizing properties, intracellular signals thought to play role during responses. found showed significant IL-8 at concentrations 10(-9) 10(-7) mol/L only after IL-5 (10 pmol/L). At these concentrations, PF-4, NAP-2, MCP-1, MIP-1 induced no priming. Unprimed response (10(-6) mol/L). Changes [Ca2+]i addition alpha, NAP-2 nmol/L) unprimed eosinophils. (10(-9) mol/L) significantly both primed eosinophils, whereas 10(-8) (10(-8) MCP-1 did not affect polymerization. findings further evidence for hypothesis cytokines like locally secreted basis specific eosinophil influx into allergic locus.