Time of day influences cytokine and clock gene response to immune stimulation in equine whole blood

摘要: Summary The immune system demonstrates regularly recurring rhythmic variation that has important medical and veterinary clinical implications. Emerging evidence supports the existence of a bidirectional immune–circadian relationship during homeostatic challenge. In peripheral blood, circadian rhythmicity manifests in terms clock gene expression, circulating levels function leucocyte cell populations cytokine production. The involvement interleukin 6 (IL6) transition from innate to acquired immunity is known, inflammatory mediators can, turn, phase shift molecular clocks. This study provides an initial investigation diurnal mediator response antigenic challenge horse. Blood samples were collected at 4-h intervals times (CT) 0, 4, 8, 12, 16 20 healthy Thoroughbred fillies maintained under winter photoperiod. Heparinized blood was cultured 37 °C for 6 h with or without lipolysaccharide (LPS) (1 μg/ml). Cells harvested by addition PAXgene™ RNA stabilizer solution immediately preserve profile. Analyses Taqman qPCR expression data reveal significant CT X LPS interaction canonical genes PER2, CRY1, ARNTL, NR1D2 (P < 0.001, P < 0.05, P < 0.01 respectively) immunomodulatory IL6 (P < 0.0001). upregulated IL1B (P < 0.01), but there no effect CT. These results confirm differentially responds over 24-h cycle, impacting on our understanding pathophysiology responses. Further research this area should provide valuable information regarding optimum time drug delivery vaccination.