Construction and analysis of tag single nucleotide polymorphism maps for six human-mouse orthologous candidate genes in type 1 diabetes

摘要: One strategy to help identify susceptibility genes for complex, multifactorial diseases is map disease loci in a representative animal model of the disorder. The nonobese diabetic (NOD) mouse human type 1 diabetes. Linkage and congenic strain analyses have identified several NOD Idd (insulin dependent diabetes) loci, which been mapped small chromosome intervals, orthologous regions genome can be identified. Here, we conducted re-sequencing association analysis six loci: NRAMP1/SLC11A1 (orthologous Nramp1/Slc11a1 Idd5.2), FRAP1 Frap1 Idd9.2), 4-1BB/CD137/TNFRSF9 4-1bb/Cd137/Tnrfrsf9 Idd9.3), CD101/IGSF2 Cd101/Igsf2 Idd10), B2M B2m Idd13) VAV3 Vav3 Idd18). Re-sequencing total 110 kb DNA from 32 or 96 diabetes cases yielded 220 single nucleotide polymorphisms (SNPs). Sixty-five SNPs, including 54 informative tag microsatellite were selected genotyped up 1,632 families 1,709 1,829 controls. None candidate showed evidence with (P values > 0.2), indicating that common variation these key does not play major role European ancestry populations studied.