Detection of Aspergillus DNA in Cerebrospinal Fluid from Patients with Cerebral Aspergillosis by a Nested PCR Assay

摘要: Invasive aspergillosis (IA), a complication with high mortality rates, especially in disseminated IA cerebral involvement, is difficult to diagnose. Biopsy of lesions often not feasible, and culture Aspergillus spp. from cerebrospinal fluid (CSF) frequently negative. New molecular methods have emerged for diagnosing IA. So far, there are only few reports DNA detection CSF. After modifying the extraction protocol, we detected CSF samples by previously described nested PCR assay. In six patients hematologic malignancy aspergillosis, were investigated DNA. was classified according EORTC/MSG 2002 criteria. Two each had proven, probable, possible Thirty-five PCR. Samples positive results assay quantified LightCycler Fourteen showed Of these, gave real-time The range CFU per ml 2,154 63,100,000. highest number found sample patient acute lymphocytic leukemia probable aspergillosis. Detection thus has potential improve diagnosis Further prospective studies larger numbers must be performed evaluate clinical significance samples.