The PAC-3 transcription factor critically regulates phenotype-associated genes in Neurospora crassa.

摘要: Transcription factors play an important role in fungal environmental adaptive process by promoting adjustment to challenging stimuli via gene modulation and activation of signaling networks. The transcription factor encoded the pac-3/rim101/pacC is involved pH regulation associated with a wide variety cellular functions. deletion pac-3 affects development. In Neurospora crassa, Δpac-3 strain presents diminished aerial growth reduced conidiation. However, PAC-3-regulated genes this altered phenotype have not been elucidated. study, we used RNA-seq analyze phenotypic plasticity induced after filamentous fungus N. crassa cultivated media supplemented sufficient or limited inorganic phosphate. Genes related morphology, hyphal development, conidiation were particular interest study. Our results suggest dependency Pi-dependent manner. Furthermore, our analysis suggested that attempts overcome effects mutant through complex combined regulatory mechanism. Finally, modulatory responses observed strain, double generated based on Δmus-52 strain-specific, highlighting impact may be attributed absence despite mus-52 deletion.