Community structure of Archaea and Bacteria in a profundal lake sediment Lake Kinneret (Israel).

摘要: Abstract The microbial community structure of an anoxic profundal lake sediment, i.e., subtropical Lake Kinneret, was analysed with respect to its composition by culture-independent molecular methods including terminal restriction fragment length polymorphism (T-RFLP) analysis, comparative sequence and quantitative real-time PCR. In particular we were interested in the structure, species composition, relative abundance overall methanogenic sediment layer (0–10 cm depth). Pairwise comparison archaeal bacterial 16S rRNA gene T-RFLP profiles obtained from three independent samplings indicated stability community. numbers Archaea Bacteria, quantified PCR, amounted about 108 1010 copies cm−3 respectively, suggesting that may account for only a minor fraction (approximately 1%) total prokaryotic Hydrogenotrophic Methanomicrobiales acetoclastic Methanosaeta spp. dominated Deltaproteobacteria, sulphate reducers syntrophs particular. second most abundant group assigned Bacteroidetes–Chlorobi-group. Only one group, which affiliated halorespiring bacteria subphylum II Chloroflexi, showed variation within samples investigated. Our study gives comprehensive insight into indicating reducers, syntrophs, bacteroidetes, halorespirers methanogens are importance Kinneret sediment.