Role of subnano-, nano- and submicron-surface features on osteoblast differentiation of bone marrow mesenchymal stem cells
作者： Dongwoo Khang , Jungil Choi , Yeon-Min Im , Youn-Jeong Kim , Je-Hee Jang
DOI: 10.1016/J.BIOMATERIALS.2012.05.005
关键词:
摘要: Subnano, nano and sub-micron surface features can selectively activate integrin receptors induce osteoblast differentiation of bone marrow mesenchymal stem cells. Although it is widely accepted that nanoscale titanium roughness may promote various lineages, there has been no clear report on the threshold dimension optimized dimensions for triggering activation cell differentiation. This study systematically controlled from sub-nano to scales investigated corresponding effects responses, such as activation, cyclins, key transcriptional genes osteoblastic phenotype genes. Surface with were insufficient increase compared pure features. both nano-submicron hybrid sufficient activating integrin-ligand proteins interactions through α subunits, only significantly accelerated subsequent primary mouse stromal cells after 2 weeks. In addition, live analysis human transparent demonstrated rapid cytoskeletal re-organization features, which ultimately induced higher expression 3
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sci-hub.se 参考文章(34)
Jin-Woo Park, Youn-Jeong Kim, Je-Hee Jang, Hwangjun Song, Osteoblast response to magnesium ion‐incorporated nanoporous titanium oxide surfaces Clinical Oral Implants Research. ,vol. 21, pp. 1278- 1287 ,(2010) , 10.1111/J.1600-0501.2010.01944.X
Dongwoo Khang, Jing Lu, Chang Yao, Karen M. Haberstroh, Thomas J. Webster, The role of nanometer and sub-micron surface features on vascular and bone cell adhesion on titanium. Biomaterials. ,vol. 29, pp. 970- 983 ,(2008) , 10.1016/J.BIOMATERIALS.2007.11.009
Manus Jonathan Paul Biggs, R Geoff Richards, Matthew J Dalby, None, Nanotopographical modification: a regulator of cellular function through focal adhesions Nanomedicine: Nanotechnology, Biology and Medicine. ,vol. 6, pp. 619- 633 ,(2010) , 10.1016/J.NANO.2010.01.009
Junichi Takagi, Konstantin Strokovich, Timothy A Springer, Thomas Walz, Structure of integrin α5β1 in complex with fibronectin The EMBO Journal. ,vol. 22, pp. 4607- 4615 ,(2003) , 10.1093/EMBOJ/CDG445
Frank Lüthen, Regina Lange, Petra Becker, Joachim Rychly, Ulrich Beck, J.G. Barbara Nebe, The influence of surface roughness of titanium on β1- and β3-integrin adhesion and the organization of fibronectin in human osteoblastic cells Biomaterials. ,vol. 26, pp. 2423- 2440 ,(2005) , 10.1016/J.BIOMATERIALS.2004.07.054
Dongwoo Khang, Sung Yeol Kim, Peishan Liu-Snyder, G. Tayhas R. Palmore, Stephen M. Durbin, Thomas J. Webster, Enhanced fibronectin adsorption on carbon nanotube/poly(carbonate) urethane: Independent role of surface nano-roughness and associated surface energy Biomaterials. ,vol. 28, pp. 4756- 4768 ,(2007) , 10.1016/J.BIOMATERIALS.2007.07.018
Allison L. Berrier, Kenneth M. Yamada, Cell–matrix adhesion Journal of Cellular Physiology. ,vol. 213, pp. 565- 573 ,(2007) , 10.1002/JCP.21237
Jessica Thomas, Biomaterials: Close to the bone Nature Nanotechnology. ,vol. 1, pp. 99- 99 ,(2006) , 10.1038/NNANO.2006.118
Matthias P. Lutolf, Penney M. Gilbert, Helen M. Blau, Designing materials to direct stem-cell fate Nature. ,vol. 462, pp. 433- 441 ,(2009) , 10.1038/NATURE08602
Barbara D Boyan, Thomas W Hummert, David D Dean, Zvi Schwartz, Role of material surfaces in regulating bone and cartilage cell response Biomaterials. ,vol. 17, pp. 137- 146 ,(1996) , 10.1016/0142-9612(96)85758-9