The Two Proteins Pat1p (Mrt1p) and Spb8p Interact In Vivo, Are Required for mRNA Decay, and Are Functionally Linked to Pab1p
作者： Claire Bonnerot , Ronald Boeck , Bruno Lapeyre
DOI: 10.1128/MCB.20.16.5939-5946.2000
关键词:
摘要: We report here the characterization of a bypass suppressor pab1Delta which leads to fourfold stabilization unstable MFA2 mRNA. Cloning wild-type gene for that reveals it is identical PAT1 (YCR077c), whose product was reported interact with Top2p. not an essential gene, but its deletion thermosensitive phenotype. Further analysis has shown allelic mrt1-3, mutation previously affect decapping and suppress pab1Delta, as also case dcp1, spb8, mrt3. Coimmunoprecipitation experiments show Pat1p associated Spb8p. On sucrose gradients, two proteins cosediment fractions containing polysomes. In absence Pat1p, however, Spb8p no longer cofractionates polysomes, while removal sharp decrease in level Pat1p. Our results suggest some factors involved mRNA degradation could be still being translated, awaiting specific signal commit pathway.
sci-hub.se 参考文章(51)
S. Z. Tarun, A. B. Sachs, Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G. The EMBO Journal. ,vol. 15, pp. 7168- 7177 ,(1996) , 10.1002/J.1460-2075.1996.TB01108.X
B.F. Horazdovsky, S.D. Emr, The VPS16 gene product associates with a sedimentable protein complex and is essential for vacuolar protein sorting in yeast. Journal of Biological Chemistry. ,vol. 268, pp. 4953- 4962 ,(1993) , 10.1016/S0021-9258(18)53488-8
M Foiani, A M Cigan, C J Paddon, S Harashima, A G Hinnebusch, GCD2, a translational repressor of the GCN4 gene, has a general function in the initiation of protein synthesis in Saccharomyces cerevisiae. Molecular and Cellular Biology. ,vol. 11, pp. 3203- 3216 ,(1991) , 10.1128/MCB.11.6.3203
B. Séraphin, Sm and Sm-like proteins belong to a large family: identification of proteins of the U6 as well as the U1, U2, U4 and U5 snRNPs. The EMBO Journal. ,vol. 14, pp. 2089- 2098 ,(1995) , 10.1002/J.1460-2075.1995.TB07200.X
F Felici, G Cesareni, J M Hughes, The most abundant small cytoplasmic RNA of Saccharomyces cerevisiae has an important function required for normal cell growth. Molecular and Cellular Biology. ,vol. 9, pp. 3260- 3268 ,(1989) , 10.1128/MCB.9.8.3260
A B Sachs, R W Davis, R D Kornberg, A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability Molecular and Cellular Biology. ,vol. 7, pp. 3268- 3276 ,(1987) , 10.1128/MCB.7.9.3268
Oscar Puig, Berthold Rutz, B. G. Mattias Luukkonen, Stefanie Kandels-Lewis, Elisabeth Bragado-Nilsson, Bertrand Séraphin, New constructs and strategies for efficient PCR‐based gene manipulations in yeast Yeast. ,vol. 14, pp. 1139- 1146 ,(1998) , 10.1002/(SICI)1097-0061(19980915)14:12<1139::AID-YEA306>3.0.CO;2-B
R.Daniel Gietz, Sugino Akio, New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene. ,vol. 74, pp. 527- 534 ,(1988) , 10.1016/0378-1119(88)90185-0
S. Z. Tarun, S. E. Wells, J. A. Deardorff, A. B. Sachs, Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translation Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 9046- 9051 ,(1997) , 10.1073/PNAS.94.17.9046
Clare A. Beelman, Audrey Stevens, Giordano Caponigro, Thomas E. LaGrandeur, Lianna Hatfield, David M. Fortner, Roy Parker, An essential component of the decapping enzyme required for normal rates of mRNA turnover. Nature. ,vol. 382, pp. 642- 646 ,(1996) , 10.1038/382642A0