Characterisation of circadian rhythms of various duckweeds.

摘要: The plant circadian clock controls various physiological phenomena that are important for adaptation to natural day–night cycles. Many components of the have been identified in Arabidopsis thaliana, model molecular genetic studies. Recent studies revealed evolutionary conservation green plants. Homologues clock-related genes isolated from Lemna gibba and aequinoctialis, it has demonstrated these homologues function system a manner similar their functioning Arabidopsis. While widely conserved, display diversity even within genus. In order survey full extent rhythms among duckweed plants, we characterised by employing semi-transient bioluminescent reporter system. Using particle bombardment method, reporters were introduced into nine strains representing five species: Spirodela polyrhiza, Landoltia punctata, gibba, L. aequinoctialis Wolffia columbiana. We then monitored luciferase (luc+) activities driven AtCCA1, ZmUBQ1 or CaMV35S promoters under entrainment free-running conditions. Under entrainment, AtCCA1::luc+ showed diurnal all strains. This suggests mechanism biological timing cycles is conserved throughout evolution duckweeds. conditions, observed AtCCA1::luc+, ZmUBQ1::luc+ CaMV35S::luc+. These period length sustainability, suggesting mechanisms somewhat diversified