Comparative proteomic analysis of Staphylococcus aureus strains with differences in resistance to the cell wall-targeting antibiotic vancomycin.
作者： Rembert Pieper , Christine L. Gatlin-Bunai , Emmanuel F. Mongodin , Prashanth P. Parmar , Shih-Ting Huang
关键词:
摘要: Three isogenic strains derived from a clinical vancomycin-intermediate Staphylococcus aureus isolate were examined by comparative protein abundance analysis. Subcellular fractionation was followed separation in 2-DE gels and spot identification MALDI-TOFTOF-MS LC-MS/MS. Sixty-five significant changes determined. Numerous enzymes participating the purine biosynthesis pathway dramatically increased strain VP32, which featured highest minimal inhibitory concentration for vancomycin, compared to P100 HIP5827. Peptidoglycan hydrolase LytM (LytM) SceD protein, putative transglycosylase, cell envelope fraction of whereas enzyme D-Ala-D-Ala ligase decreased its cytosol fraction. Furthermore, penicillin-binding 2 (PBP2) had substantially higher activity VP32 that LytM, PBP2 catalyze reactions or metabolism wall peptidoglycan. It is plausible expression these are responsible an altered turnover rate, has been observed, peptidoglycan structure, yet be elucidated this highly vancomycin-resistant strain.
elsevier.com
sci-hub.se 参考文章(47)
Bashkirov Vi, Mil'shina Nv, Prozorov Aa, [Nucleotide sequence and physical map of kanamycin-resistant plasmid pUB110 from Staphylococcus aureus]. Genetika. ,vol. 22, pp. 1081- 1092 ,(1986)
H Hara, S Yasuda, K Horiuchi, J T Park, A promoter for the first nine genes of the Escherichia coli mra cluster of cell division and cell envelope biosynthesis genes, including ftsI and ftsW. Journal of Bacteriology. ,vol. 179, pp. 5802- 5811 ,(1997) , 10.1128/JB.179.18.5802-5811.1997
N Mani, P Tobin, R K Jayaswal, Isolation and characterization of autolysis-defective mutants of Staphylococcus aureus created by Tn917-lacZ mutagenesis. Journal of Bacteriology. ,vol. 175, pp. 1493- 1499 ,(1993) , 10.1128/JB.175.5.1493-1499.1993
R K Jayaswal, Y I Lee, B J Wilkinson, Cloning and expression of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity. Journal of Bacteriology. ,vol. 172, pp. 5783- 5788 ,(1990) , 10.1128/JB.172.10.5783-5788.1990
Krzysztof Sieradzki, Alexander Tomasz, Gradual Alterations in Cell Wall Structure and Metabolism in Vancomycin-Resistant Mutants of Staphylococcus aureus Journal of Bacteriology. ,vol. 181, pp. 7566- 7570 ,(1999) , 10.1128/JB.181.24.7566-7570.1999
Ryan T Cirz, Jodie K Chin, David R Andes, Valérie de Crécy-Lagard, William A Craig, Floyd E Romesberg, Inhibition of mutation and combating the evolution of antibiotic resistance. PLOS Biology. ,vol. 3, ,(2005) , 10.1371/JOURNAL.PBIO.0030176
Linda M Weigel, Don B Clewell, Steven R Gill, Nancye C Clark, Linda K McDougal, Susan E Flannagan, James F Kolonay, Jyoti Shetty, George E Killgore, Fred C Tenover, Genetic Analysis of a High-Level Vancomycin-Resistant Isolate of Staphylococcus aureus Science. ,vol. 302, pp. 1569- 1571 ,(2003) , 10.1126/SCIENCE.1090956
Anatoly Severin, Keiko Tabei, Fred Tenover, Marilyn Chung, Nancy Clarke, Alexander Tomasz, High level oxacillin and vancomycin resistance and altered cell wall composition in Staphylococcus aureus carrying the staphylococcal mecA and the enterococcal vanA gene complex. Journal of Biological Chemistry. ,vol. 279, pp. 3398- 3407 ,(2004) , 10.1074/JBC.M309593200
D M Shlaes, J H Shlaes, S Vincent, L Etter, P D Fey, R V Goering, Teicoplanin-resistant Staphylococcus aureus expresses a novel membrane protein and increases expression of penicillin-binding protein 2 complex. Antimicrobial Agents and Chemotherapy. ,vol. 37, pp. 2432- 2437 ,(1993) , 10.1128/AAC.37.11.2432
Rita Napolitano, Regine Janel‐Bintz, Jerome Wagner, RPP Fuchs, All three SOS-inducible DNA polymerases (Pol II, Pol IV and Pol V) are involved in induced mutagenesis. The EMBO Journal. ,vol. 19, pp. 6259- 6265 ,(2000) , 10.1093/EMBOJ/19.22.6259