MAP kinase activity is downregulated by phorbol ester during mouse oocyte maturation and egg activation in vitro.

摘要: The effects of protein kinase C (PKC) stimulator, phorbol 12-myriatate 13-acetate (PMA), on meiotic cell cycle regulation and mitogen-activated (MAP) changes have been studied in mouse oocytes eggs. results showed that MAP activation itself was not necessary for germinal vesicle breakdown (GVBD), but the ability ooplasm to phosphorylate a prerequisite this event. At concentrations 1.6 nM, PMA effectively inhibited GVBD activation, suggesting inhibits by inhibiting molecule(s) upstream kinase. 16.2 induced metaphase-interphase transition more eggs collected 19 hr after human chorionic gonadotropin (hCG) administration than those 15 hCG administration. degree activity decrease well correlated with time course proportion pronuclear formation. On other hand, when effect progression abolished phosphatase inhibitor, okadaic acid, superactivated. biologically inactive 4 alpha-phorbol 12,13-didecanoate (4 alpha-PDD) had no evident either interphase or activity. Furthermore, oocyte GVBD, egg could be overcome specific PKC calphostin C, possible involvement enzyme suggest entrains cascade events ultimately induces inactivation