Unveiling the Structural Basis That Regulates the Energy Transduction Properties within a Family of Triheme Cytochromes from Geobacter sulfurreducens

摘要: A family of triheme cytochromes from Geobacter sulfurreducens plays an important role in extracellular electron transfer. In addition to their transfer pathways, two members this (PpcA and PpcD) were also found be able couple e–/H+ through the redox Bohr effect observed physiological pH range, a feature not for PpcB PpcE. attempting understand molecular control cytochromes, which is highly homologous both amino acid sequence structures, it was that residue 6 conserved leucine PpcA PpcD, whereas other characterized (PpcB PpcE) equivalent phenylalanine. To determine located close center, we replaced Leu6 with Phe determined properties mutant, as well its solution structure fully reduced state. contrast native form, muta...