The nucleotide sequence of the structural gene for Escherichia coli tryptophanyl-tRNA synthetase.

摘要: Abstract The complete nucleotide sequence of trpS, the structural gene for Escherichia coli tryptophanyl-tRNA synthetase, was determined using a plasmid carrying gene. From single open reading frame correct length and orientation we deduced an amino acid consistent with composition purified protein. In addition, previously sequenced peptides representing 52% protein were readily aligned regions sequence. E. enzyme is 60% homologous from Bacillus stearothermophilus. Using currently available procedures predicted secondary structure each organism compared these structures to those two aminoacyl-tRNA synthetases whose three-dimensional have been determined. We used convenient recombination procedure map regional locations missense mutations within trpS that characteristic effects on properties enzyme.