MicroRNAs that target RGS5.

摘要: Objective(s):An earlier meta-analysis on gene expression data derived from four microarray, two cDNA library, and one SAGE experiment had identified RGS5 as of only ten non-housekeeping genes that were reported to be expressed in human trabecular meshwork (TM) cells by all studies. encodes regulator G-protein signaling-5. The TM tissue is the route aqueous fluid outflow, relevant pathology glaucoma. MicroRNAs constitute most recently components cellular machinery for regulation eukaryotic cells. Given our long standing interest glaucoma, we aimed identify miRNAs may target RGS5. Materials Methods: Eight selected study using bioinformatics tools available eye. Their effects assessed dual luciferase assay.  3'-UTR segments mRNA cloned downstream a coding psiCHECK2 vectors, these co-transfected with each into HEK293 Results: outcomes evidenced or more are fact targeted miR-7, miR-9, miR-96, miR-23a, miR-23b, miR-204, miR-211. Down regulations statistically significant. effect miR-204 considered particularly important this miRNA well known regulate eye development affect multiple ocular functions. Conclusion: Our results justify further studies functions miRNAs.