Comparative analysis of neutrophil and monocyte epigenomes
作者： Daniel Rico , Joost HA Martens , Kate Downes , Enrique Carrillo-de-Santa-Pau , Vera Pancaldi
DOI: 10.1101/237784
关键词:
摘要: Neutrophils and monocytes provide a first line of defense against infections as part the innate immune system. Here we report integrated analysis transcriptomic epigenetic landscapes for circulating neutrophils with aim to enable downstream interpretation functional validation key regulatory elements in health disease. We collected RNA-seq data, ChIP-seq six histone modifications DNA methylation by bisulfite sequencing at base pair resolution from up 6 individuals per cell type. Chromatin segmentation analyses suggested that have higher number cell-specific enhancer regions (4-fold) compared neutrophils. This highly plastic epigenome is likely indicative greater differentiation potential into macrophages, dendritic cells osteoclasts. In contrast, most neutrophil-specific features tend be characterized repressed chromatin, reflective their status terminally differentiated cells. Enhancers were where differences between observed, monocyte-specific enhancers being generally hypomethylated. Monocytes show substantially gene expression levels than neutrophils, epigenomic revealing more active monocytes. Our suggest overexpression c-Myc its binding could an important contributor these differences. Altogether, our study provides comprehensive chart chromatin states primary human monocytes, thus providing valuable resource studying regulation
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