Molecular characterization of new clinical isolates of Candida albicans and C. dubliniensis in Japan: analysis reveals a new genotype of C. albicans with group I intron.
作者： M. Tamura , K. Watanabe , Y. Mikami , K. Yazawa , K. Nishimura
DOI: 10.1128/JCM.39.12.4309-4315.2001
关键词:
摘要: The genetic diversity of recent clinical isolates Candida albicans in Japan was studied on the basis amplified DNA band lengths determined with a specific PCR primer reported to have been designed span transposable intron region 25S rRNA gene. Our analyses 301 C. showed that they could be classified into five genotypes: genotype A (172 isolates), B (66 C (56 D (C. dubliniensis; 5 and new (designated E; 2 isolates). E characterized group I intron-like sequence, which is longer than hitherto ones has nucleotide sequence length 962 bp. analysis 962-bp indicated it composed an similar dubliniensis 621 bp 341-bp insertion. Analysis internal transcribed spacer (ITS) strain its identical those strains other genotypes, only few base substitution differences. Throughout study, possible horizontal transfer between suggested. high degree correlation presence susceptibility antifungal agent flucytosine observed. examined present study when were compared by randomly polymorphic fingerprinting pattern analysis, this also confirmed ITS sequences.
sci-hub.se 参考文章(21)
Erika Bruck, National Committee for Clinical Laboratory Standards. Pediatrics. ,vol. 65, pp. 187- 188 ,(1980)
Natteewan Poonwan, Tamae Imai, Nanthawan Mekha, Katsukiyo Yazawa, Yuzuru Mikami, Akikazu Ando, Yoshiho Nagata, Genetic Analysis of Histoplasma capsulatum Strains Isolated from Clinical Specimens in Thailand by a PCR-Based Random Amplified Polymorphic DNA Method Journal of Clinical Microbiology. ,vol. 36, pp. 3073- 3076 ,(1998) , 10.1128/JCM.36.10.3073-3076.1998
Cletus P. Kurtzman, Christie J. Robnett, Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. ,vol. 73, pp. 331- 371 ,(1998) , 10.1023/A:1001761008817
Uwe von Ahsen, Julian Davies, Renée Schroeder, Antibiotic inhibition of group I ribozyme function. Nature. ,vol. 353, pp. 368- 370 ,(1991) , 10.1038/353368A0
Ste`phane Mercure, Serge Montplaisir, Guy Lernay, Correlation between the presence of a self-splicing intron in the 25S rDNA of C.albicans and strains susceptibility to 5-fluorocytosine Nucleic Acids Research. ,vol. 21, pp. 6020- 6027 ,(1993) , 10.1093/NAR/21.25.6020
W. R. Pearson, D. J. Lipman, Improved tools for biological sequence comparison. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 85, pp. 2444- 2448 ,(1988) , 10.1073/PNAS.85.8.2444
K. KAMEI, M. J. MCCULLOUGH, D. A. STEVENS, Initial case of Candida dubliniensis infection from Asia: non-mucosal infection. Medical Mycology. ,vol. 38, pp. 81- 83 ,(2000) , 10.1080/MMY.38.1.81.83
Uwe von Ahsen, Reneée Schroeder, Streptomycin inhibits splicing of group I introns by competition with the guanosine substrate Nucleic Acids Research. ,vol. 19, pp. 2261- 2265 ,(1991) , 10.1093/NAR/19.9.2261
R. Maleszka, G. D. Clark-Walker, Yeasts have a four-fold variation in ribosomal DNA copy number. Yeast. ,vol. 9, pp. 53- 58 ,(1993) , 10.1002/YEA.320090107
Julie D. Thompson, Desmond G. Higgins, Toby J. Gibson, CLUSTAL W: IMPROVING THE SENSITIVITY OF PROGRESSIVE MULTIPLE SEQUENCE ALIGNMENT THROUGH SEQUENCE WEIGHTING, POSITION-SPECIFIC GAP PENALTIES AND WEIGHT MATRIX CHOICE Nucleic Acids Research. ,vol. 22, pp. 4673- 4680 ,(1994) , 10.1093/NAR/22.22.4673