Comparisons of eight commercial on-farm milk progesterone tests.

摘要: Abstract To evaluate eight commercial on-farm milk progesterone kits, samples (50 ml each of foremilk and postmilk strippings) were collected during the estrous cycle from 10 cycling Holstein cows for 24 consecutive days. Relative concentrations classified as low or high by comparison with standard supplied kit. The concentration in sample was determined radioimmunoassay (RIA). Accuracy classification into levels tests percentage similarity RIA values using discriminant analysis. ranged 89.0 to 98.9% progesterone, 74.8 85.6% 80.3 87.3% all (n = 238). fat an interaction milkfat day influenced test results except Accufirm Calfcheck. Progesterone levels, estimated test-kits, 1.5 ± 0.5 2.8 0.9 days before estrus (X SEM) until 4.0 0.6 5.9 1.3 after estrus. These data support principle that a single cannot be used determine proper timing insemination. All kits can accurately relative samples.